论文部分内容阅读
目的探讨长链非编码RNA(lncRNA)锌指E盒结合同源盒蛋白(ZEB)2-AS1在Ⅲ期大肠癌中的表达及意义。方法诊断为Ⅲ期大肠癌并行手术切除患者87例,采用实时定量PCR法检测其大肠癌组织及癌旁正常黏膜组织中lncRNA ZEB2-AS1的表达,分析其与临床病理参数及预后的关系。结果大肠癌组织中lncRNA ZEB2-AS1表达较癌旁正常黏膜组织增高18.75倍(P<0.01),其增高与患者死亡相关(P<0.01)。LncRNA ZEB2-AS1高表达(≥381.5)组5年生存率低于低表达(<381.5)组(43.2%vs.76.7%)(P<0.01)。LncRNA ZEB2-AS1表达、淋巴结分期和肿瘤部位是Ⅲ期大肠癌患者预后的独立影响因素(P<0.05或P<0.01)。结论 LncRNA ZEB2-AS1参与大肠癌的发生和发展,是影响预后的独立因素。
Objective To investigate the expression and significance of long-chain non-coding RNA (lncRNA) zinc finger E-box binding and homeobox (ZEB) 2-AS1 in stage III colorectal cancer. Methods Eighty-seven patients with stage III colorectal cancer were surgically resected. The expression of lncRNA ZEB2-AS1 in colorectal cancer tissue and adjacent normal mucosa was detected by real-time quantitative PCR. The relationship between lncRNA ZEB2-AS1 and clinicopathological parameters and prognosis was analyzed. Results The expression of lncRNA ZEB2-AS1 in colorectal cancer tissue was increased by 18.75 fold (P<0.01) compared with normal mucosa around the tumor, and the increase was associated with death (P<0.01). The 5-year survival rate of LncRNA ZEB2-AS1 high-expression (≥381.5) group was lower than that of low-expression (<381.5) group (43.2% vs.76.7%) (P<0.01). LncRNA ZEB2-AS1 expression, lymph node staging, and tumor site were independent prognostic factors in patients with stage III colorectal cancer (P<0.05 or P<0.01). Conclusion LncRNA ZEB2-AS1 is involved in the occurrence and development of colorectal cancer and is an independent factor that influences prognosis.