结核病是严重危害人民健康的常见病之一。用PCR方法检测临床标本中的结核杆菌DNA可以快速、敏感、特异地诊断结核病。我科采用在结核杆菌中重复出现的DNA片段为靶DNA,用PCR技术进行扩增,引物的DNA核苷酸顺序为5’CCTGC GAGCG TAGGC GTCGG 3’和5’CTCGT CCAGCGCCGC TTCGG 3’。在反应中用94℃变性,68℃退火、延伸,43个循环可以将1pg TBDNA扩增到 Tuberculosis is one of the common diseases that seriously endanger people’s health. PCR detection of clinical specimens of M. tuberculosis DNA can be rapid, sensitive and specific diagnosis of tuberculosis. In our department, the DNA fragment repeated in Mycobacterium tuberculosis was used as target DNA and amplified by PCR. The DNA sequence of the primer was 5’CCTGC GAGCG TAGGC GTCGG 3 ’and 5’CTCGT CCAGCGCCGC TTCGG 3’. 1 pg of TBDNA was amplified in 43 reactions by denaturation at 94 ° C, annealing at 68 ° C, extension of 43 cycles