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目的研究细胞因子对肾癌细胞特异性抗原G250表达的影响。方法单独或联合应用不同浓度的白细胞介素(IL)-2、干扰素(IFN)-α、IFN-γ刺激肾癌786-0细胞系,刺激24、48、96 h后,运用免疫组织化学、Western blot和逆转录-聚合酶链反应(RT-PCR)技术检测G250抗原在肾癌786-0细胞上的表达。结果IL-2、IFN-α、IFN-γ均能上调786-0细胞G250抗原表达,以IFN-γ作用最强,上调作用具有浓度及时间依赖性。细胞因子联用能明显增强G250抗原表达的上调作用。结论IL-2、IFN-α、IFN-γ能上调肾癌786-0细胞G250抗原表达,联合应用效果更大。提示细胞因子联合以G250抗原为靶点的免疫治疗有望成为晚期肾癌新的治疗途径。
Objective To study the effect of cytokines on G250 expression of renal cell carcinoma antigen. Methods The renal cell carcinoma 786-0 cells were stimulated with different concentrations of interleukin (IL) -2, interferon (IFN) -α and IFN-γ separately or in combination for 24, 48 and 96 h. Immunohistochemistry The expression of G250 antigen on 786-0 cells was detected by Western blot and reverse transcription-polymerase chain reaction (RT-PCR). Results Both IL-2, IFN-α and IFN-γ upregulated the G250 antigen expression in 786-0 cells. The effect of IFN-γ was the strongest and the up-regulation effect was concentration-dependent and time-dependent. Cytokines can significantly enhance the G250 antigen expression upregulation. Conclusion IL-2, IFN-α and IFN-γ can up-regulate the G250 antigen expression in 786-0 human renal cell carcinoma, which is more effective in combination. Prompted cytokines combined with G250 antigen as the target of immunotherapy is expected to become a new treatment for advanced renal cell carcinoma.