目的:建立快速测定硫酸妥布霉素注射液含量的方法。方法:采用旋光法进行测定。结果:浓度在2～6万u·ml~(-1)范围内与旋光度呈线性关系,平均回收率99.9％,RSD0.4％(n=5),与微生物法一致。结论:方法简便、快速、准确,尤适用于药厂、医院对该制剂的质量控制和快速分析。 Objective: To establish a rapid method for the determination of tobramycin sulfate injection. Methods: The optical rotation method was used for determination. Results: The linear relationship was obtained with the optical rotation in the range of 2 ~ 60 000 u · ml -1. The average recovery was 99.9% and the RSD was 0.4% (n = 5), which was consistent with the microbial method. Conclusion: The method is simple, rapid and accurate, especially suitable for the quality control and rapid analysis of the preparation by the pharmaceutical factory and the hospital.