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目的:通过脑脊液蛋白质组学实验技术体系,筛选并鉴定帕金森病患者脑脊液特异蛋白,初步探讨帕金森病的发病机制。方法:选自广州医学院第一附属医院神经内科与脑深部电刺激术(deep brainstimulation,DBS)治疗中心帕金森病患者和对照组脑脊液为研究对象,进行双向凝胶电泳,经银染、图像分析寻找差异蛋白质,并进行质谱分析鉴定。结果:鉴定得到4种差异蛋白质,分别是CENP-E、Tetranectin非钙离子C型凝集素样结构域、假想蛋白FLJ38159和KIAA1640。帕金森病组中KIAA1640蛋白含量占总蛋白含量的比例(0.09±0.05)%高于对照组(0.03±0.00)%,CENP-E(0.04±0.02)%、Tetranectin非钙离子C型凝集素样结构域(0.04±0.02)%、假想蛋白FLJ38159(0.11±0.06)%蛋白含量均低于对照组[(0.08±0.01)%,(0.07±0.02)%,(0.17±0.04)%],P<0.05。结论:本研究鉴定出4种差异蛋白,这些蛋白可能参与帕金森病的发病,但具体机制及其在帕金森病发病中的作用有待进一步研究。
OBJECTIVE: To screen and identify cerebrospinal fluid specific proteins in patients with Parkinson’s disease through cerebrospinal fluid proteomics experimental system and to explore the pathogenesis of Parkinson’s disease. Methods: Cerebrospinal fluid (CSF) of Parkinson’s disease patients and control group was collected from Department of Neurology and the Department of Neurosurgery, the First Affiliated Hospital of Guangzhou Medical College, and deep brain stimulation (DBS) for two-dimensional gel electrophoresis. The silver staining, Analysis Find differences in proteins, and identified by mass spectrometry. RESULTS: Four differential proteins were identified, which were CENP-E, Tetranectin non-calcium C-type lectin-like domains, hypothetical proteins FLJ38159 and KIAA1640. The percentage of KIAA1640 protein in the Parkinson’s disease group was (0.09 ± 0.05)% higher than that in the control group (0.03 ± 0.00)%, CENP-E (0.04 ± 0.02)%, Tetranectin non-calcium ion C-type lectin (0.08 ± 0.01)%, (0.07 ± 0.02)%, (0.17 ± 0.04)%], P <0.05, respectively, in the control group (0.04 ± 0.02)% and the hypothetical protein FLJ38159 0.05. Conclusion: Four different proteins were identified in the present study. These proteins may be involved in the pathogenesis of Parkinson’s disease. However, the specific mechanisms and their roles in the pathogenesis of Parkinson’s disease are yet to be further studied.