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目的 定量比较研究小鼠经中子及γ线照射后肠道损伤的病理特点。方法 350只二级雄性BALB/C小鼠,经不同剂量的中子和γ线照射,于照后6h、12h、1d、2d、3d、4d、5d、7d、10d、14d、21d及28d分批活杀,进行全肠长度及重量测量;全肠组织10%缓冲福尔马林固定,石蜡包埋制片, 潘氏细胞及HE染色,Leica图像分析仪检测隐窝细胞数密度和隐窝内潘氏细胞的面密度。结果 中子及γ线照射后全肠的长度缩短、重量减轻,且重量减轻先于长度缩短。2.5Gy中子照射后肠粘膜见明显损伤及损伤后恢复现象,4.0Gy以上照射未见明显恢复。g 射线5.5Gy照射未见粘膜剥脱,12Gy组隐窝再生能力介于中子4.0-5.5Gy之间。中子2.5Gy照后1d内,隐窝细胞数进行性减少,于照后3d增多,7d达高峰,而5.5Gy中子和12Gyγ线于照后3d内均明显进行性减少(p<0.01)。γ线5.5Gy隐窝细胞增加发生时间早,高峰提前。中子2.5Gy照后7d时潘氏细胞明显增加(p<0.05);而5.5Gy组于照后2d潘氏细胞相对增多(p<0.05)。5.5Gyγ线于照后5d增加(p<0.05)。结论 照射后肠隐窝细胞明显损伤,相同剂量照射后,中子对隐窝细胞损伤明显重于γ线;射线对潘氏细胞影响较小,相同剂量的中子及γ线照射后,潘氏细胞出现不同的变化规律。
Objective To quantitatively compare the pathological characteristics of intestinal tract injury induced by neutron and γ ray in mice. Methods 350 male BALB / C mice were irradiated with different doses of neutron and γ ray at 6h, 12h, 1d, 2d, 3d, 4d, 5d, 7d, 10d, 14d, 21d and 28d The total intestine was fixed in 10% buffered formalin, paraffin-embedded, Paneth and HE stained. The number of crypt cells and crypts were detected by Leica image analyzer The surface area of Papanicolaou cells. Results After neutron and γ ray irradiation, the length of the whole intestine was shortened, the weight was reduced, and the weight loss was shortened before the length. After 2.5Gy neutron irradiation, the intestinal mucosa was found to be obviously damaged and recovered after injury. No obvious recovery was found after 4.0Gy irradiation. g-ray 5.5Gy no mucosal exfoliation, 12Gy group crypt regeneration ability between neutrons 4.0-5.5Gy. The number of crypt cells decreased progressively after 1 day of 2.5Gy neutron irradiation, increased 3d on day 3 and peaked on 7d, while 5.5Gy neutron and 12Gy line decreased significantly after 3d (p <0.01) . Gamma line 5.5Gy crypt cells increased early, peak early. Panell cells increased significantly at 7 days after 2.5Gy neutron irradiation (p <0.05), while Paneth cells increased at 2 days after irradiation in 5.5Gy group (p <0.05). 5.5 Gy line increased 5d after irradiation (p <0.05). Conclusions After irradiation, the intestinal crypt cells are obviously damaged. At the same dose of irradiation, the neutrons damage the crypt cells significantly more than the γ-ray; the radiation has little effect on the PAN cells. After the same dose of neutron and γ-ray irradiation, Cells appear different changes.