论文部分内容阅读
33只成年雄兔行双侧输精管结扎手术后,进行连续12个月的免疫学观察。7只同龄雄兔作为空白对照。结果表明,间接血凝试验69.7%结扎组家兔检测出抗精子抗体,滴度范围为1:5～1:1280;间接免疫荧光测定有90.9%的实验组家兔测出抗精子抗体。白细胞粘附抑制实验呈阳性反应者占实验组家兔的43.5%,与对照组比较差异显著。应用PEG光密度和抗补体法测定CIC均里阴性。输精管结扎后第3个月附睾肿胀者占45%,第5—7个月达70%,之后逐渐消退。而与此同时抗精子抗体阳性检出率明显增高,故可推断精子抗原主要经附睾入血,作用于免疫系统导致体液与细胞免疫反应。结扎组家兔胸腺、脾、淋巴结呈明显的增生现象支持这一结论。
Thirty-three male adult rabbits underwent bilateral vasectomy and performed immunological observations for 12 consecutive months. Seven male rabbits of the same age were used as blank controls. The results showed that anti-sperm antibodies were detected in 69.7% of ligation rabbits in the indirect hemagglutination test, and the titer ranged from 1:5 to 1:1280. Indirect immunofluorescence assay showed that anti-sperm antibodies were detected in 90.9% of experimental rabbits. Leukocyte adhesion inhibitory test positive reaction accounted for 43.5% of rabbits in the experimental group, which was significantly different from the control group. The CIC was negative using PEG densitometry and anti-complement assay. After 3 months of vasectomy, 45% of the epididymal swelling occurred, and 70% of the 5th to 7th months later gradually subsided. At the same time, the positive detection rate of anti-sperm antibodies is obviously higher, so it can be inferred that sperm antigen mainly enters the blood through the epididymis and acts on the immune system to cause humoral and cellular immune responses. The obvious hyperplasia of the thymus, spleen and lymph nodes in the ligation group supported this conclusion.