目的 :为研究卵巢癌的免疫治疗方法构建合适的动物模型。方法 :严重联合免疫缺陷 (severecombinedimmunodeficiency ,SCID)小鼠皮下接种人卵巢癌细胞系SKOV3,同时腹腔注射人脾或外周血淋巴细胞进行免疫功能重建。观察成瘤情况 ;并用ELISA法检测鼠血清中人IgG含量以及流式细胞学和ABC免疫组织化学法测定鼠脾内人T细胞表型鉴定免疫重建。结果 :免疫重建的小鼠成瘤率为 89.6 % ,免疫组化证实皮下瘤保持原细胞系特征。 3种方法检测发现用人外周血淋巴细胞免疫重建的效果优于用脾淋巴细胞。结论 :免疫重建的荷人卵巢癌 SCID小鼠模型已初步建立成功 Objective: To establish a suitable animal model for the study of immunotherapy of ovarian cancer. Methods: Severely combined immunodeficiency (SCID) mice were inoculated subcutaneously with human ovarian cancer cell line SKOV3, and intraperitoneally injected with human spleen or peripheral blood lymphocytes for immune function reconstruction. The tumorigenicity was observed. The content of human IgG in serum was detected by ELISA and the phenotype of human T cells in spleen was identified by immunocytochemistry and ABC immunohistochemistry. Results: The rate of tumor formation in immunosuppressed mice was 89.6%. Immunohistochemistry confirmed that the subcutaneous tumor maintained the characteristics of the original cell line. Three kinds of methods to detect the use of peripheral blood lymphocyte immune reconstitution better than spleen lymphocytes. Conclusion: Immunohistochemical SCID mouse model of human ovarian cancer has been initially established successfully