目的研究南方地区气候条件下持物镊干燥无菌保存效果。方法在使用过程中采样,用无菌试验方法对南方某三甲医院不同季节和不同时间段干燥保存无菌持物镊进行了检验。结果使用中无菌持物镊干式保存4 h内采样检验无菌生长,保存6 h后细菌阳性率为3.7%。冬季干式保存无菌持物镊细菌阳性率明显少于其他季节;春季干式保存无菌持物镊细菌阳性率低于夏季秋季。用戊二醛保存的无菌持物镊取出后不用无菌水冲洗,在3 d内检测无菌生长;无菌持物镊取出用无菌水冲洗后使用,保存8 d内检测无菌生长。结论南方地区任何季节使用干保存无菌持物镊,只能保持4 h无菌;戊二醛溶液湿式保存可延长保存时间。 Objective To study the effect of holding sterile tweezers in aseptic conditions in southern China under climatic conditions. Methods Samples were collected during use. Sterile test methods were used to test the tweezers of aseptic holding in different seasons and different time periods in a hospital in the south of China. Results Aseptic tweezers were used for dry-in-place sampling within 4 h for aseptic growth. After 6 h of storage, the positive rate of bacteria was 3.7%. The positive rate of bacteria-holding tweezers in winter dry-keeping sterile tweezers was significantly less than that in other seasons; the positive rate of tweezers in spring dry-keeping sterile tweezers was lower than that in summer. Sterile holding tweezers stored with glutaraldehyde were removed without sterile water and tested for growth within 3 days. Sterile tweezers were removed after rinsing with sterile water and stored for up to 8 days. Conclusions In southern China, sterile storage tweezers were used for dry storage for any season, which could only be kept for 4 h. The wet storage of glutaraldehyde solution could prolong the storage time.