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目的:研究核因子-κB(NF-κB)在离体培养的人早孕期细胞滋养层细胞激活的机制。方法:离体培养人早孕期细胞滋养细胞,以白细胞介素-1β(IL-1β)和NF-κB抑制剂——吡咯烷二硫代氨基甲酸酯(PDTC)作为处理因素,通过免疫荧光检测NF-κB的亚基p65的核转位;凝胶电泳迁移率改变分析(EMSA)法检测NF-κBDNA结合活性。结果:①IL-1β作用30min后细胞滋养细胞出现了p65的核转位;②细胞滋养细胞NF-κBDNA结合活性显著增高;PDTC能显著降低NF-κBDNA结合活性。结论:IL-1β在离体培养的人早孕期细胞滋养细胞可引起NF-κB的短暂激活,PDTC可以抑制IL-1β诱导的NF-κB的激活。这为进一步研究激活或抑制NF-κB是否能调节NF-κB的靶基因、尤其是在胚胎着床和妊娠发挥重要作用的靶基因提供了理论基础。
Objective: To investigate the mechanism of nuclear factor-κB (NF-κB) activation in cultured human trophoblasts in early pregnancy. Methods: Cytotrophoblast cells were cultured in vitro in human first trimester. IL-1β and PDTC were used as the treatment factors. The expression of interleukin-1β (IL-1β) The nuclear translocation of NF-κB subunit p65 was detected. The EMSA method was used to detect the NF-κB DNA binding activity. RESULTS: ① The nuclear translocation of p65 was found in cytotrophoblasts after IL-1β treatment for 30min. ② The NF-κB DNA binding activity of cytotrophoblasts was significantly increased. PDTC could significantly decrease the NF-κB DNA binding activity. CONCLUSION: IL-1β can induce transient activation of NF-κB in cultured human pre-pregnancy cytokines and PDTC can inhibit IL-1β-induced activation of NF-κB. This provides a theoretical basis for further research on whether activating or inhibiting NF-κB can regulate target genes of NF-κB, especially target genes that play an important role in embryo implantation and pregnancy.