目的　为获得人Ⅱ型囊泡单胺转运体 (vesicularmonominetransporter 2VMAT2 )以治疗帕金森病 ,克隆添加Kozak序列的VMAT2cDNA ,用于真核细胞表达。方法　从人胎脑中提取总RNA ,逆转录成cDNA ,设计一对引物用PCR方法扩增目的cDNA片段并将其重组于 pGEM Easy T载体中 ,酶切鉴定插入片段后 ,进行全序列测定。结果　RT PCR扩增到 16 37bp的带有Kozak序列的cDNA片段。结论　克隆人VMAT2cDNA片断 ,可用于真核细胞表达 Objective To obtain vesicular monomine transporter 2 VMAT2 for the treatment of Parkinson’s disease, VMAT2 cDNA with Kozak sequence was cloned for eukaryotic expression. Methods Total RNA was extracted from human fetal brain and reversely transcribed into cDNA. A pair of primers was designed to amplify the target cDNA fragment by PCR and recombined into pGEM Easy T vector. The inserted fragment was digested with restriction endonucleases and sequenced. Results The 16 37 bp cDNA fragment with Kozak sequence was amplified by RT-PCR. Conclusion Human VMAT2 cDNA fragment can be expressed in eukaryotic cells