论文部分内容阅读
目的研究ＬＡＫ细胞的活性诱导和杀伤机制。方法用ＭＴＴ法检测不同培养时间的ＬＡＫ细胞、ＬＡＫ细胞冻融液及培养上清液对肿瘤细胞杀伤作用的相关性以及对肿瘤的杀伤作用。方法加入终浓度为１ｍｇ／Ｌ的放线菌素Ｄ以阻断检测过程中肿瘤增殖对实验结果影响。结果①ＬＡＫ细胞冻融液和培养上清液的杀伤活性与ＬＡＫ细胞活性呈正相关（ｒ分别为０．９９６和０．９９９，直线相关性ｔ检验Ｐ＜０．００１和０．００５）；②动态杀伤分析显示，ＬＡＫ细胞动态杀伤活性与ＬＡＫ细胞冻融液、培养上清液之间不呈直线性相关（ｒ分别为０．８４７，０．７９９，Ｐ值均＞０．０５），而ＬＡＫ细胞冻融液和培养上清液间则呈显著直线相关（ｒ为０．９６９，Ｐ＜０．０１）。这表明ＬＡＫ细胞对肿瘤细胞的杀伤方式为快速杀伤，而ＬＡＫ细胞冻融液和培养上清液对肿瘤细胞的杀伤方式则为慢杀伤，ＬＡＫ细胞杀伤活性明显高于ＬＡＫ细胞冻融液。结论ＬＡＫ细胞活性诱导与胞浆内细胞毒因子表达有关；ＬＡＫ细胞可能通过释放细胞毒因子对肿瘤细胞起直接杀伤作用；ＬＡＫ细胞通过与肿瘤细胞直接接触释放杀伤介质，而导致肿瘤细胞凋亡，是其高效、快速杀伤肿瘤细胞的作用机制。
Objective To study the activity induction and killing mechanism of LAK cells. Methods The MTT assay was used to detect the correlation between the killing effect of LAK cells, LAK cell freeze-thaw fluid and culture supernatant on tumor cells and the killing effect on tumor cells. Methods Actinomycin D was added at a final concentration of 1 mg/L to block the effects of tumor proliferation on the experimental results. Results 1 The killing activity of LAK cell freeze-thawing fluid and culture supernatant was positively correlated with the activity of LAK cells (r = 0.996 and 0.999 respectively, linear correlation t-test P<0.001 and 0.005); 2 dynamics The killing analysis showed that the dynamic killing activity of LAK cells was not linearly related to the freeze-thaw solution of LAK cells and culture supernatant (r = 0.847, 0.799, P>0.05), while LAK There was a significant linear correlation between cell freeze-thaw and culture supernatant (r = 0.969, P < 0.01). This shows that LAK cells kill tumor cells in a rapid manner, while LAK cell freeze-thawing fluid and culture supernatants kill tumor cells in a slow killing manner. LAK cell killing activity is significantly higher than LAK cell freezing and thawing. Conclusion The induction of LAK cell activity is related to cytoplasmic cytotoxic factor expression. LAK cells may directly kill tumor cells by releasing cytotoxic factors; LAK cells, through direct contact with tumor cells, release the killing medium and cause apoptosis of tumor cells. It is the mechanism of its efficient and rapid killing of tumor cells.