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为了确定大麦籽粒产量和苗期耐低氮能力与小孢子培养阶段的氮胁迫下愈伤组织产量之间是否存在一致性,以2份大麦基因型为供试材料,进行了以下3项研究:(1)培养基中水解干酪素和谷氨酰胺使用量对小孢子培养愈伤组织产量的影响;(2)营养液中NH4NO3不同添加量对大麦苗期生长量的影响;(3)盆栽时正常施氮与不施氮处理对大麦单株产量的影响。结果表明,培养基中氮含量的下降明显降低小孢子培养愈伤组织产量,2份基因型的降幅存在明显的差异;营养液中氮使用量的下降明显降低植株高度、主根长度和植株及根干重,2份基因型之间的降幅也存在明显的差异;盆栽时不施氮处理的大麦有效穗和单株产量低于正常施氮的对照,2份基因型间有差异。氮素胁迫下,2份基因型小孢子培养愈伤组织产量的相对值与苗期植株高度、茎叶干重、主根长度和根干重的相对值以及单株产量的相对值存在一致性,说明供试基因型小孢子水平与植株水平的耐低氮性存在相关性。
In order to determine whether there is consistency between the grain yield of barley and the ability of low nitrogen tolerance at seedling stage and the callus production under nitrogen stress in microspore culture stage, two barley genotypes were used as materials for the following three studies: (1) the effect of hydrolyzed casein and glutamine on callus production of microspore culture in medium; (2) the effect of different NH4NO3 addition in nutrient solution on the growth of barley seedling; (3) Effect of Normal and Non - nitrogen Fertilization on Yield of Single Barley. The results showed that the decrease of nitrogen content in the medium significantly reduced the callus production of microspore culture, and there was a significant difference in the decline of two genotypes. The decrease of nitrogen use in nutrient solution significantly reduced the plant height, length of main root and plant and root There was also a significant difference between the two genotypes in dry weight and in the two genotypes. The effective spike and yield per plant of pots without nitrogen application were lower than those with normal nitrogen application, and there were differences among the two genotypes. Under nitrogen stress, the relative values of callus production of two microsatellite cultivars were consistent with the relative values of plant height, stem and leaf dry weight, main and root dry weight and relative yield per plant, This indicated that there was a correlation between the microspore level of the tested genotypes and the low nitrogen tolerance level of the plants.