由胆固醇与1，6-己二异氰酸酯反应合成了1，6-己二异氰酸酯胆固醇单酯（Ⅰ），然后通过葡聚糖与（Ⅰ）反应合成了具有自聚集性质的葡果糖，测得葡聚糖主链上每100个糖环含有3－5个胆固醇．我们采用探针式超声波来制得胆固醇修饰葡聚糖［CHD］的水溶液，同时用N-苯基-α-萘胺[PNA]作为荧光探针来研究CHD自聚集过程．在实验中我们发现，每一种葡聚糖溶液，都存在着一个临界浓度．在此浓度之上，PNA的荧光屏强度大大增强，表明CHD分子中的胆固醇自聚集而形成了疏水性胶束状的聚集体． 1,6-Hexamethylene diisocyanate cholesteryl ester (I) was synthesized by the reaction of cholesterol and 1,6-hexamethylene diisocyanate, and then the glucan was synthesized by the reaction of dextran with (Ⅰ) The glycan backbone contains 3-5 cholesterol per 100 sugar rings. We used probe-based ultrasound to prepare an aqueous solution of cholesterol-modified dextran [CHD] and studied the CHD self-assembly process using N-phenyl-alpha-naphthylamine [PNA] as a fluorescent probe. In the experiment we found that there is a critical concentration for each dextran solution. Above this concentration, the fluorescence intensity of the PNA is greatly enhanced, indicating that the CHD molecules self-aggregate to form hydrophobic micellar aggregates.