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根据hBLyS (humanBlymphocytestimulator)基因序列设计合成特异性引物 ,用RT_PCR从人外周血淋巴细胞扩增出 85 8bp的hBLyS基因 ,并将其插入到融合蛋白原核表达载体 pGEX_4T_1中 ,得到重组表达质粒pGEX_4T_1/hBLyS。把此重组质粒转化大肠杆菌BL2 1,经用IPTG诱导 ,表达出了GST_hBLyS融合蛋白。
According to the hBLyS gene sequence, 858bp hBLyS gene was amplified from human peripheral blood lymphocytes by RT_PCR and inserted into the prokaryotic expression vector pGEX_4T_1 to get the recombinant plasmid pGEX_4T_1 / hBLyS . The recombinant plasmid was transformed into E. coli BL21 and induced by IPTG to express GST_hBLyS fusion protein.