我所实验动物研究室,于1982年5月从北京中医研究院引进Wistar远交系大鼠,并以此为基础建成一封闭群,目前种群达700只左右。在常规剖验中,发现二例自发恶性肿瘤,作了大体解剖,并将病变器官用10％福尔马林固定,取组织块作石蜡包埋、切片,经伊红-苏木素染色,作了组织学观察。第一例: 1.大体解剖:在饲养种群中,发现一只10周龄雌性大鼠呼吸粗重,行动迟缓,无食欲,第二天死亡。剖验发现:胸腔内充满清亮淡黄积液,两侧肺脏均与胸膈膜、肺纵隔及心脏粘连。大部分肺组织形成大小不等的葡萄状肿块,总体积2.5×3.5×3.5cm~3(图1)。右肺肿块侵入横膈膜。其它器官未见明显异常。取肺肿瘤组织作病理切片。 2.镜检结果:瘤细胞大小及形状极不一致,呈多 My laboratory animal laboratory, in May 1982 from the Beijing Institute of Chinese medicine to introduce Wistar outbred rats, and as a basis for the formation of a closed group, the current population of about 700. In a routine dissection test, two cases of spontaneous malignancy were found, grossly dissected, and diseased organs were fixed with 10% formalin. Tissue pieces were paraffin-embedded, sectioned and stained with eosin-hematoxylin Histological observation. First case: 1. Gross anatomy: In a breeding herd, a 10-week-old female rat was found to have heavy breathing, slow motion, no appetite and died the following day. Anatomy found that: the chest is filled with bright yellow fluid, both sides of the lung and chest diaphragm, pulmonary mediastinal and cardiac adhesions. Most of the lung tissue to form the size of the grape-like mass, the total volume of 2.5 × 3.5 × 3.5cm ~ 3 (Figure 1). Right lung mass invades the diaphragm. Other organs no obvious abnormalities. Take lung tumor tissue for biopsy. 2. Microscopy results: the size and shape of tumor cells are extremely inconsistent, were more