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目的研究人工发酵的斜生褐孔菌菌质对链脲佐菌素(STZ)致2型糖尿病大鼠的影响,并与野生斜生褐孔菌菌核进行比较。方法高糖、高脂饲料饲养SD大鼠6周,单次ip给予STZ35mg/kg制备2型糖尿病模型。将模型大鼠随机分成模型组、斜生褐孔菌菌质(菌质)1g/kg组、野生斜生褐孔菌菌核(菌核)1g/kg组和盐酸二甲双胍(0.2g/kg)阳性对照组。连续ig给药10周后,检测各组大鼠体质量以及空腹血糖、胰岛素、糖化血红蛋白(HbA1c)、血清游离脂肪酸(FFA)、总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白-胆固醇(HDL-C)、低密度脂蛋白-胆固醇(LDL-C)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)等水平,并进行口服葡萄糖耐量实验(OGTT)。结果给药10周后,与模型组比较,菌质组和菌核组大鼠的体质量明显增加(P<0.05),血糖明显降低(P<0.05),血清FFA、TC、TG和MDA水平下降(P<0.05),SOD、GSH-Px水平升高(P<0.05);菌质组与菌核组的上述指标无显著差异。结论斜生褐孔菌菌质能缓解STZ致2型糖尿病大鼠症状,具有降低血糖、调节血脂、改善氧化应激的作用,且作用强度与野生斜生褐孔菌菌核相当。
Objective To study the effect of artificial fermentation of stomatal fungi on the type 2 diabetic rats induced by streptozotocin (STZ), and to compare with the sclerotium of wild P. oblique fungi. Methods SD rats were fed with high-sucrose and high-fat diet for 6 weeks, and type 2 diabetes mellitus was induced by STZ 35 mg / kg ip. The model rats were randomly divided into model group, 1g / kg group of Stomatal fungi (1 g / kg group), 1g / kg wild scleractinia sclerotiorum (sclerotium) and metformin hydrochloride (0.2g / Positive control group. After continuous ig administration for 10 weeks, the body weight, fasting blood glucose, insulin, HbA1c, FFA, TC, TG, HDL HDL-C, LDL-C, SOD, MDA, GSH-Px, And other levels, and oral glucose tolerance test (OGTT). Results After 10 weeks of administration, compared with the model group, the body weight of the rats in the bacterial group and the sclerotiorum group was significantly increased (P <0.05), the blood glucose was significantly decreased (P <0.05), and the levels of FFA, TC, TG and MDA (P <0.05), and the levels of SOD and GSH-Px increased (P <0.05). There was no significant difference in the above indexes between the bacterial group and the sclerotia group. CONCLUSION: Stramenopore fungi can alleviate the symptoms of STZ-induced type 2 diabetic rats, and have the effects of lowering blood sugar, regulating blood fat and improving oxidative stress. The strength of the fungi is comparable to that of the wild S. sclerotiorum.