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采用DE52柱层析法部分纯化大鼠中的蛋白激酶C，研究了石上柏和蒲葵子醇提物对蛋白激酶C活性的影响。为了证明测定系统的可靠性，观察了多粘菌素B（已知蛋白激酶C抑制剂）对蛋白激酶C活性的影响。表明：石上柏醇提物对蛋白激酶C有强烈的抑制作用，IC502．2μg／ml；100μg／ml的蒲葵子醇提物对蛋白激酶C的抑制率为66．6％，降低药物浓度抑制作用减弱；多粘菌素B对蛋白激酶C有抑制作用，IC5017．7U／ml。
Partial purification of protein kinase C in rats by DE52 column chromatography was performed to investigate the effects of ethanol extracts of P. schrenkiana and P. apetala on protein kinase C activity. To demonstrate the reliability of the assay system, the effect of polymyxin B (a known protein kinase C inhibitor) on protein kinase C activity was observed. The results showed that the ethanol extract of P. ostreatus had a strong inhibitory effect on protein kinase C, IC502.2 μg/ml; the inhibitory effect of 100 μg/ml P. korshinskii ethanol extract on protein kinase C was 66.6%, which reduced the inhibition of drug concentration. Weakened; polymyxin B inhibited protein kinase C, IC5017.7U/ml.