有关胆汁酸的分离和测定已有不少报告。国外报告的HPLC法,甘氨结合型胆汁酸需被做成p溴苯酯后在254 nm检测,前处理复杂。国内报告的HPLC法,其中牛磺二羟基异构体胆汁酸的分离欠佳,本文目的是建立操作简便、分离效果好、精密度高的HPLC测八种结合型胆汁酸的方法。 There have been many reports on the separation and determination of bile acids. According to the HPLC method reported by foreign countries, the glycine ammonia-conjugated bile acid is required to be detected at 254 nm after preparation of p-bromophenyl ester, and the pretreatment is complicated. The domestically reported HPLC method, in which the isolation of the bile acids of the tauro-dihydroxy isomers is not good, the purpose of this article is to establish a HPLC method for the determination of eight bound bile acids that is easy to operate, with good separation and high precision.