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目的探讨在空间记忆中丘脑前核细胞内信号转导是否与MAPK途径有关。方法成年雄性SD大鼠34只,分为3组:①PD组:侧脑室内注射PD098059溶液;②PD对照组;③正常组。在Morris水迷宫中,定位航行训练后,行侧脑室内注射,24h后空间探索实验。免疫组化和Western-Blot检测p-ERK的变化。结果在空间探索实验中PD组寻找平台次数及在靶象限停留时间与PD对照组及正常对照组相比明显减少(p<0.01)。在p-ERK免疫组化和Western-Blot中,PD组与PD对照组比较,丘脑前核内p-ERK表达明显减弱,光密度分析有显著性差异(p<0.01)。在PD对照组,丘脑前背侧核与丘脑前腹侧核相比,p-ERK表达显著增强,光密度分析有显著性差异(p<0.05)。结论丘脑前核空间记忆细胞内信号转导可能与MAPK途经有关。
Objective To investigate whether signal transduction in pre-thalamic nuclei in spatial memory correlates with MAPK pathway. Methods Thirty-four adult male Sprague-Dawley rats were divided into three groups: ① PD group: intracerebroventricular injection of PD098059 solution; ② PD control group; ③ normal group. In the Morris water maze, positioning sailing training, intracerebroventricular injection, space exploration experiment 24h. Immunohistochemistry and Western-Blot detection of p-ERK changes. Results In space exploration experiment, the number of platform searching for PD group and the dwell time in target quadrant decreased significantly compared with PD control group and normal control group (p <0.01). In p-ERK immunohistochemistry and Western-Blot, p-ERK expression in the thalamic nucleus significantly decreased compared with the PD control group, and the optical density analysis showed significant difference (p <0.01). In the PD control group, p-ERK expression was significantly increased in the thalamic anterior dorsal nucleus compared to the thalamic anterior ventral nucleus, and there was a significant difference in optical density (p <0.05). Conclusion The signal transduction in spatial memory of thalamic nucleus may be related to the pathway of MAPK.