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目的探讨α肾上腺素对大鼠重症急性胰腺炎(SAP)模型中肺脏水调节能力的影响。方法 15只SD大鼠随机分为假手术组和SAP组,SAP组根据造模后取材的不同时相分为SAP 4 h组和SAP 24 h两个亚组,每组5只大鼠。测定各组大鼠肺脏的肺水清除率(AFC),然后进一步应用α1肾上腺素抑制剂prazosin和α2肾上腺素抑制剂yohimbine处理SAP组大鼠后再观察AFC的变化。用real-time PCR方法测定α1和α2肾上腺素受体m RNA在各组大鼠肺脏组织中的表达。结果 1各组肺脏湿干比变化:与假手术组比较,SAP组4 h(P<0.05)和24 h(P<0.05)时的肺脏湿干比均明显下降;与SAP组4 h时比较,SAP组24 h时的肺脏湿干比进一步降低(P<0.05)。2 AFC变化:SAP组大鼠肺脏在4 h和24 h时的AFC均明显高于假手术组(P<0.05),但SAP组24 h时较4 h时轻度下降,但差异无统计学意义(P>0.05)。经过α1肾上腺素受体抑制剂prazosin或α2肾上腺素受体抑制剂yohimbine作用后,其AFC较SAP组相应时相均明显下降(P<0.05),但仍明显高于假手术组(P<0.05);经prazosin和yohimbine联合作用后,其AFC较SAP组、SAP+prazosin组和SAP+yohimbine组相应时相均明显下降(P<0.05),几乎完全抑制到假手术组的水平(P>0.05)。3 SAP组4 h和24 h时,大鼠肺脏组织中α1和α2肾上腺素受体m RNA表达水平均明显高于假手术组(P<0.05),但SAP组24 h时较SAP组4 h时仅轻度下降,二者比较差异无统计学意义(P>0.05)。结论本研究初步研究结果提示,α1、α2肾上腺素受体均可能参与了大鼠SAP时肺脏水的调节。
Objective To investigate the effects of α-adrenaline on lung water-regulating capacity in rats with severe acute pancreatitis (SAP). Methods Fifteen SD rats were randomly divided into sham operation group and SAP group. The SAP group was divided into SAP 4 h group and SAP 24 h group according to the different phases of the model. Five rats in each group. Pulmonary water clearance rate (AFC) of rats in each group was measured. The changes of AFC in SAP group were further observed after treatment with α1-epinephrine inhibitor prazosin and α2-epinephrine inhibitor yohimbine. Real-time PCR was used to determine the expression of α1 and α2 adrenergic receptor mRNA in the lungs of rats in each group. Results 1 The changes of lung wet-dry ratio in each group: Compared with the sham operation group, the wet / dry ratio of lung in the SAP group decreased significantly at 4 h (P <0.05) and 24 h (P <0.05) , And the wet / dry ratio of lungs in SAP group decreased further at 24 h (P <0.05). Changes of AFC: The AFC of lungs in SAP group at 4 and 24 h was significantly higher than that in sham operation group (P <0.05), but the SAP group slightly decreased at 24 h compared with 4 h, but the difference was not statistically significant Significance (P> 0.05). After treated with α1-adrenoceptor inhibitor prazosin or α2-adrenoceptor inhibitor yohimbine, AFC was significantly lower than that of SAP group (P <0.05), but still significantly higher than that of sham operation group (P <0.05) ). The combination of prazosin and yohimbine significantly decreased the AFC level compared with the SAP group, the SAP + prazosin group and the SAP + yohimbine group (P <0.05), and almost completely suppressed the level of AFC in the sham operation group (P> 0.05 ). Compared with the sham-operated group, the mRNA expression levels of α1 and α2 adrenergic receptors in the lung tissue of SAP group were significantly increased at 4 h and 24 h (P <0.05) When only mild decline, the difference between the two was not statistically significant (P> 0.05). Conclusion The preliminary results of this study suggest that both α1 and α2 adrenergic receptors may participate in the regulation of lung water in rat SAP.