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The reason why chlorsulfuron (2-chloro-N-[4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl]benzenesulfonamide)bound residues can stillmake an injury to rotational crops is still keptunknown. The experiment was conducted under laboratory conditions to determine thedynamics ofextractable and non-extractable (bound) residues of chlorsulfuron in soil, and the distribution ofchlorsulfuron bound residues in organic matter fractions. The resultsshowed that extractable 14C-residuesdecreased to 25.12% of applied chlorsulfuron over an incubation period of 150 days; this in turn, theformation of bound residues increased to 47.07% of the applied. Theproportions of 14C-bound residues in soilorganic matter fractions increase in order of: humic acid(HA)