AIM:TO clarify the association of vascular endothelialgrowth factor (VEGF) and microvascular density (MVD)expression with the angiogenesis and prognosis ofcolorectal cancer.METHODS:A total of 97 cases of colorectal carcinomaswere examined by immunohistochemical staining (SPmethod),using anti-VEGF and anti-factor CD34~ monoclonalantibodies.RESULTS:VEGF positive staining was obtained in 68 outof 97 cases (70.1%),and observed mainly in the cytoplasmof tumor cells,and also frequently in stromal cells.VEGFexpression was more intense in poorly differentiatedadenocarcinoma in comparison with others,but there wasno significant correlation between VEGF expression and age,sex and stage.A significant correlation was found betweenthe MVD and grades,and there was no significant relationshipbetween the MVD and age,sex,and stage.The MVD in theVEGF positive group (68 cases) was higher than that in thenegative group.Upon multivariate analysis,the significantvariables were stage,tumor grade and MVD;VEGFexpression was not an independent prognostic factor.CONCLUSION:The expression of VEGF has a significantcorrelation with MVD;MVD expression has prognostic valuebut VEGF has not in colon cancer. AIM: TO clarify the association of vascular endothelial growth factor (VEGF) and microvascular density (MVD) expression with the angiogenesis and prognosis of colorectal cancer. METHODS: A total of 97 cases of colorectal carcinomaswere examined by immunohistochemical staining (SPmethod), using anti-VEGF and anti-factor CD34 ~ monoclonalantibodies.RESULTS: VEGF positive staining was obtained in 68 out of 97 cases (70.1%), and observed mainly in the cytoplasm of tumor cells, and also frequently in stromal cells. VEGF expression was more intense in poorly differentiated adenocarcinoma in comparison with others, but there wasno significant correlation between VEGF expression and age, sex and stage. A significant correlation was found betweenthe MVD and grades, and there was no significant relationshipbetween the MVD and age, sex, and stage.The MVD in theVEGF positive group (68 cases) was higher than that in the negative group .Upon multivariate analysis, the significant variables were stage, tumor grade and MVD; VEGF expression was not an independent prognostic factor. CONCLUSION: The expression of VEGF has a significantcorrelation with MVD; MVD expression has prognostic valuebut VEGF has not in colon cancer.