在我国水稻育种中,迄今还没有一种比较适用的蛋白质筛选技术,致使高蛋白育种工作的进行有一些困难。因此我们从水稻高蛋白育种的需要出发,进行了三硝基苯磺酸(TNBS)染色法测定籽粒蛋白质含量的研究,取得了初步结果。这一测定技术简单、快速,只需10—20粒种子就能测出蛋白质含量,而且不破坏种子的发芽力,是分析水稻品种高蛋白较适用的筛选技术,从而为水稻高蛋白育种创造了条件。 In our country rice breeding, so far there is no more suitable protein screening technology, resulting in the progress of high protein breeding has some difficulties. Therefore, based on the need of rice high protein breeding, we conducted a study on the determination of grain protein content by TNBS staining and achieved preliminary results. This assay technique is simple and rapid, only 10-20 seeds can measure the protein content, and does not undermine the seed germination force, is a more suitable screening technology for analysis of high-protein rice varieties, thus creating a high-protein breeding of rice condition.