论文部分内容阅读
目的研究分析临床感染患者分离的肠球菌属细菌耐药性及耐药基因,为临床治疗提供参考依据。方法收集2012年医院分离的383株肠球菌属进行菌株鉴定和敏感试验,并对284株多药耐药菌株进行耐药分析及基因检测,数据采用SPSS 17.0软件进行统计分析。结果 383株肠球菌属中屎肠球菌和粪肠球菌位居前两位,分别占59.5%和34.2%;284株多药耐药菌株中阳性基因TEM、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ant(6)-Ⅰ、ant(2″)-Ⅰ、tetM、ermB,检出率依次为77.5%、62.3%、26.1%、13.4%、36.6%、31.3%、66.5%,粪肠球菌和屎肠球菌耐药基因阳性率不同,而同一种细菌其耐药表型与耐药基因型一致。结论临床分离的肠球菌属多药耐药性严重,携带抗菌药物相关耐药基因是导致菌株对抗菌药物产生耐药的重要原因,因此加强对细菌耐药基因的检测,对肠球菌属的感染治疗具有重要的意义。
Objective To study the bacterial resistance and drug resistance genes of enterococci isolated from patients with clinical infection and provide reference for clinical treatment. Methods 383 strains of enterococci isolated from hospitals in 2012 were collected for strain identification and sensitivity test. 284 multidrug-resistant strains were analyzed for drug resistance and genetic testing. The data were analyzed by SPSS 17.0 software. RESULTS: Enterococcus faecium and Enterococcus faecalis were the top two among 383 Enterococcus species, accounting for 59.5% and 34.2% respectively. The positive genes TEM, aac (6 ’) / aph (2 “ The detection rates of aph (3 ’) - Ⅲ, ant (6) - Ⅰ, ant (2 ”) - Ⅰ, tetM and ermB were 77.5%, 62.3%, 26.1%, 13.4%, 36.6% and 31.3 %, 66.5%, Enterococcus faecalis and Enterococcus faecium resistant gene positive rate is different, and the same bacteria resistant phenotype and resistant genotypes. Conclusions The clinical isolates of Enterococcus are multidrug-resistant with serious antibiotic resistance-related genes, which is one of the most important causes for the resistance of strains to antibacterials. Therefore, the detection of drug-resistant genes in Escherichia coli, Treatment is of great importance.