对RAPD标记在合浦珠母贝F1代全同胞家系共27个样品中的分离方式进行了研究。从21条RAPD随机引物中筛选了8条引物用于扩增分析,结果共扩增出49个位点,其中正常位点47个(95.9%),异常位点2个(4.1%),即子代有扩增带而双亲无。47个正常位点中3个位点在F1中不分离(6.4%),44个位点发生分离(93.6%,即多态位点)。卡方检验表明,44个分离位点中32个符合孟德尔分离规律(72.7%),12个位点发生偏分离(27.3%)。符合孟德尔分离位点中按11分离位点占43.8%,31分离位点占56.2%,其中,父本特有标记、母本特有标记和双亲共有标记分别占28.2%,15.6%和56.2%,父本特有标记略多于母本特有标记。根据“双假测交”理论,11分离位点是构建F1代家系的作图位点。较高的11分离位点表明,利用RAPD标记构建合浦珠母贝遗传连锁图谱是可行的。 The separation of RAPD markers in a total of 27 samples of F1 progeny of Pombe pekinensis family was studied. Eight primers were screened from 21 RAPD random primers for amplification. The results showed a total of 49 loci, of which 47 (95.9%) were normal and 2 (4.1%) were abnormal Progeny with amplification and parents without. Three of the 47 normal loci were not segregated in F1 (6.4%), and 44 loci (93.6%, ie polymorphic loci) were segregated. Chi-square test showed that 32 out of 44 isolated sites met Mendel’s law (72.7%) and 12 isolated sites (27.3%). According to the Mendelian isolates, 43.8% of the isolates were segregated at 11 and 56.2% of the isolates at 31. Among them, 28.2% and 15.6% were male-specific markers, female-specific markers and parents’ And 56.2% respectively. There were slightly more special markers of male parents than female ones. According to the theory of “double fake test”, the 11 segregation site is the mapping site for constructing F1 generation pedigree. The higher 1: 1 segregation locus indicates that it is feasible to construct the genetic linkage map of Pinus tabulaeformis with RAPD markers.