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利用农杆菌介导的高效遗传转化系统 ,将抗真菌r -硫堇蛋白Rs -afp1基因导入黄淮稻区主栽品种豫梗 6号的胚性愈伤组织 ,获得了 8株转基因植株 ,Gus染色和PCR分析表明 ,外源基因已整合到水稻基因组中
Using Agrobacterium - mediated transformation system with high efficiency, the antifungal r - thiovin protein Rs - afp1 gene was introduced into the embryogenic callus of Yulong 6, a major cultivar of Huanghuai rice area. Eight transgenic plants, Gus Staining and PCR analysis showed that foreign genes have been integrated into the rice genome