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目的探讨弓形虫感染对子一代雄性小鼠发育期脑部多巴胺水平的影响。方法 36只雌性ICR小鼠随机分为健康对照组和弓形虫感染组,每组18只。感染组每鼠分别经口感染弓形虫PRU弱毒株10个包囊。感染后90 d,与健康雄性ICR小鼠按1∶1交配。每组各取2只交配成功的母鼠于孕20 d时剖腹取胎鼠。其他交配成功的母鼠自然分娩。应用高效液相色谱-电化学法对孕20 d的雄性胎鼠及出生后14 d和63 d的雄性仔鼠(各6只)进行脑皮层、小脑、海马和纹状体多巴胺含量的测定。结果感染组小鼠感染后死亡3只,余15只感染鼠中,仅6只交配成功。2只于孕20 d剖腹取胎鼠12只,其中雄性7只;4只自然分娩,仔鼠存活21只,其中雄性15只。18只对照组小鼠均交配成功,2只于孕20 d时剖腹取胎鼠23只,其中雄性12只;16只自然分娩,仔鼠存活179只,其中雄性92只。感染组和对照组雄性胎鼠小脑区多巴胺含量分别为(413.25±21.78)ng/g和(346.30±51.83)ng/g,感染组显著高于对照组(P<0.01),两者皮层区多巴胺含量差异无统计学意义(P>0.05)。感染组出生后14 d和63 d,雄性仔鼠皮层区[(462.50±24.80)ng/g和(1 215.77±113.64)ng/g]、小脑区[(271.55±26.19)ng/g和(1 328.82±39.62)ng/g]、海马区[(225.78±24.17)ng/g和(1 322.70±58.34)ng/g]和纹状体区[(455.23±61.53)ng/g和(991.32±54.31)ng/g]的多巴胺含量均显著高于相应对照组(P<0.05或P<0.01)。结论弓形虫感染能引起子一代雄性小鼠发育期脑部多巴胺水平显著升高。
Objective To investigate the effect of Toxoplasma gondii infection on dopamine levels in brain of the first generation male mice during development. Methods Thirty-six female ICR mice were randomly divided into healthy control group and Toxoplasma gondii infection group, with 18 mice in each group. Infected group, 10 mice were infected with Toxoplasma gondii PRU attenuated strain. At 90 days post infection, healthy male ICR mice were mated at 1: 1. Two mated mice from each group were selected and their fetuses were taken by cesarean section on the 20th day of gestation. Other mates who successfully mate naturally give birth. The content of dopamine in the cortex, cerebellum, hippocampus and striatum of male fetus 20 days pregnant and male offspring 14 days and 63 days after birth (6 mice each) were determined by HPLC - electrochemical method. Results Infected mice died 3 after infection, and the remaining 15 infected mice, only 6 were successfully mating. Twelve fetuses were fetched from 12 caesarean sections in pregnancy, of which 7 were males. Four females were born naturally, and 21 were survived, including 15 males. Twenty-eight control mice were successfully mated. Twenty-three fetuses were obtained by intra-abdominal cesarean section on day 20 of pregnancy, of which 12 were males. Sixteen mice were born spontaneously and the offspring survived. There were 92 males. The dopamine levels in cerebellum of infected and control groups were (413.25 ± 21.78) ng / g and (346.30 ± 51.83) ng / g, respectively, significantly higher than those in control group (P <0.01) There was no significant difference in content (P> 0.05). The levels of cortical area [(462.50 ± 24.80) ng / g and (2115.77 ± 113.64) ng / g] in cerebellum and [(271.55 ± 26.19) ng / g and 328.82 ± 39.62 ng / g], [(225.78 ± 24.17) ng / g and (13232 ± 58.34) ng / g] and striatum [(455.23 ± 61.53) ng / g and (991.32 ± 54.31 ) ng / g] were significantly higher than the corresponding control group (P <0.05 or P <0.01). Conclusion Toxoplasma gondii infection can cause brain dopamine levels to increase significantly in the first generation of male mice.