论文部分内容阅读
目的探讨人脑梗死灶周围组织星形胶质细胞和微血管的变化。方法应用9例因脑梗死而死亡尸检全脑标本,常规 HE 和免疫组织化学染色(SP)标记星形胶质细胞中的胶质纤维酸性蛋白(GFAP)和微血管标记物(CD31),观察星形胶质细胞和微血管的变化规律。结果以梗死灶为中心,由内到外可分4个区(0～3区);GFAP 在0区和1区表达很少,在2区和3区随缺血时间延长表达持续升高;CD31在0区无表达,在1区可见少量表达,在2区和3区随缺血时间延长表达持续升高。结论脑梗死后星形胶质细胞和微血管反应性增生,在缺血后期 GFAP、CD31广泛表达,两者在组织损伤修复重建过程中起重要作用。
Objective To investigate the changes of astrocytes and microvessels in human peripheral cerebral infarction. Methods Nine brain autopsy specimens of cerebral infarction were collected. Glial fibrillary acidic protein (GFAP) and microvessel marker (CD31) in astrocytes were labeled by routine HE and immunohistochemical staining (SP) Glial cells and microvascular changes. Results There were 4 zones (0 ~ 3) in the infarct zone, but no expression in zone 0 and 1 in GFAP. The expression of GFAP in zones 2 and 3 continued to increase with prolonged ischemia. No expression of CD31 in the 0 area, a small amount of expression in 1 area, in 2 and 3 areas with prolonged ischemia expression continued to rise. Conclusions Astrocytes and microvessel reactive hyperplasia after cerebral infarction are widely expressed in the late phase of ischemia, both of which play an important role in the process of tissue injury repair and reconstruction.