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目的探讨卡维地洛对柯萨奇病毒B3(CVB3)病毒性心肌炎小鼠的作用。方法随机将80只小鼠分为3组:正常对照组(n=20),心肌炎组(n=30)与卡维地洛组(n=30),利用BALB/c小鼠感染CVB3建立病毒性心肌炎模型,感染24 h后每日灌胃给予卡维地洛10 mg/kg直至第14天末,分别于接种第7天和第14天随机从各组抽取若干只小鼠取血后处死并留取心脏等标本。结果卡维地洛降低病毒性心肌炎小鼠急性期的病死率,显著减轻心脏病理损伤,显著减低HW/BW(HW,心脏重量;BW,身体重量)比值;卡维地洛干预后第7天小鼠心肌IFN-γ表达水平显著高于心肌炎对照组,MDA含量显著降低;干预后第14天SOD含量显著增加,MDA含量显著降低。结论卡维地洛通过增加心肌IFN-γ表达水平与抗氧化的双重作用减轻感染小鼠的心肌损害,提高生存率,起到保护性作用。
Objective To investigate the effect of carvedilol on Coxsackievirus B3 (CVB3) viral myocarditis in mice. Methods Eighty mice were randomly divided into three groups: normal control group (n = 20), myocarditis group (n = 30) and carvedilol group (n = 30). BALB / c mice were used to infect CVB3 The model of myocarditis was induced by intragastric administration of carvedilol 10 mg / kg 24 h after the infection until the end of the 14th day. Some mice were randomly selected from each group on the 7th day and the 14th day after inoculation to take blood and sacrificed And retain the heart and other specimens. Results Carvedilol reduced the mortality of mice with viral myocarditis in the acute phase, significantly reduced the cardiac pathological injury, and significantly reduced the ratio of HW / BW (HW, heart weight; BW, body weight); on the 7th day after carvedilol intervention The expression of IFN-γ in myocardium of mice was significantly higher than that of myocarditis control group, and the content of MDA was significantly decreased. On the 14th day after intervention, the content of SOD was significantly increased while the content of MDA was significantly decreased. CONCLUSION Carvedilol may play a protective role by increasing the expression of myocardial IFN-γ and the dual role of antioxidation in reducing the myocardial damage and increasing the survival rate of infected mice.