论文部分内容阅读
微卫星锚定片段长度多态性(microsatellite-anchored fragment length polymorphism,MFLP)分子标记技术是结合AFLP和SSR双重原理创造的。本研究采用通用荧光引物M13-F-IRDye700(5’-CACGAC GTTGTAAACGAC-3’),利用MFLP分子标记技术建立了适合茶树的PCR反应体系,从324对引物体系中,筛选出6对最适合的引物对,对壶瓶山的44株茶树样品进行试验,分析其亲缘关系。6对MFLP选择性扩增引物共同扩增出288条带,其中多态性条带232条,多态性为80.6%,得出44株有性繁殖茶苗两两间的遗传相似系数在0.10~0.58之间,这表明同一地理位置,不同有性繁殖群体的基因组存在差异性。
Microsatellite-anchored fragment length polymorphism (MFLP) molecular marker technology is based on the dual principle of AFLP and SSR. In this study, a universal fluorescent primer M13-F-IRDye700 (5’-CACGACGTTGTAAACGAC-3 ’) was used to establish a PCR reaction system suitable for the tea plantation by using the MFLP molecular marker technique. Six pairs of primer pairs Primer pairs, Hupingshan 44 tea samples were tested to analyze their kinship. 6 pairs of MFLP selective amplified primers amplified 288 bands, of which 232 polymorphic bands, the polymorphism was 80.6%, obtained 44 sexual propagation of tea seedling between the two genetic similarity coefficient of 0.10 ~ 0.58, indicating that the same geographic location, sexual reproduction groups of different genomes.