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香蕉是芭蕉科芭蕉属多年生的三倍体无籽的常绿性大型草本植物。它须通过母株吸芽或地下块茎进行无性繁殖,但繁殖速度很慢。近年来,香蕉吸芽组织培养在国内外已广泛应用于生产繁殖技术。目前,本课题在该项技术基础上,进一岁研究出一种取材更方便,繁殖速度更快的香蕉花序组织培养新方法,并已获得一批幼苗,可望对香蕉的繁殖创出一条新的途径。研究是利用开花结果后的香蕉花序末端的雄花、花苞、花轴等切成碎片接种在含有不同含量的细胞分裂素、生长素的 MS 培养基上,经10天培养,雄花膨大,而苞片和花轴有75.3%的外植体变褐,甚至枯死。经30天雄花发生胚状体,40天达到高峰,发生率
Banana is a perennial triploid banana plantain seedless evergreen herb. It has to be vegetatively propagated by female plant buds or underground tubers, but reproduces slowly. In recent years, banana bud tissue culture at home and abroad have been widely used in production and breeding techniques. At present, on the basis of this technology, this project has developed a new method for tissue culture of banana inflorescences which is more convenient and reproduce faster, and has obtained a batch of seedlings, which is expected to create a banana breeding New way. The study was conducted on the MS medium containing different contents of cytokinin and auxin after inoculating male flowers, buds and flower axils at the end of the inflorescences. After 10 days of culture, the male flowers dilated while the bracts And the axis of the flower shaft with 75.3% of the browning, or even dead. After 30 days of male embryogenesis occurs, 40 days to reach the peak, the incidence