柑桔皮精油用ＫＯＨ皂化，用无水乙醚提取不皂化物，蒸去乙醚，残渣用无水乙醇溶解，超滤后取清液注入高效液相色谱仪，以反相柱分离，紫外可见分光检测器检测其中的β－胡萝卜素和维生素Ｅ。柑桔皮精油用正丁醇适当稀释后直接用平台石墨炉原子吸收法测定其中的硒。测定结果：柑桔皮精油中β－胡萝卜素，维生素Ｅ和硒的含量分别为０１６８×１０－３（ＲＳＤ＝５４％，ｎ＝５），１８１×１０－３（ＲＳＤ＝７２％，ｎ＝５）和０５５×１０－６（ＲＳＤ＝３７％，ｎ＝９）。 Citrus peel essential oil saponification with KOH, anhydrous ethyl ether unsaponifiable matter, steamed to remove ether, the residue was dissolved in anhydrous ethanol, after ultrafiltration supernatant was injected into the high performance liquid chromatography, reverse phase column separation, UV-visible spectroscopy The detector detects beta-carotene and vitamin E. Citrus peel essential oil with n-butanol, after appropriate dilution directly with the platform graphite furnace atomic absorption method for the determination of selenium. The results showed that the contents of β-carotene, vitamin E and selenium in citrus peels were 0168 × 10-3 (RSD = 5.4%, n = 5) and 181 × 10-3 (RSD = 72%, n = 5) and 055 × 10-6 (RSD = 37%, n = 9).