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观察大鼠肠缺血－再灌流过程中小肠、肝、肺组织ＴＮＦα和ＩＬ－６ｍＲＮＡ表达的规律，发现肠缺血１ｈ，小肠ＴＮＦα和ＩＬ－６ｍＲＮＡ表达开始增加，再灌流后０．５～１ｈ表达至峰值，并且与组织及血浆中蛋白水平变化趋势一致；与小肠相比，肝、肺组织ＴＮＦα和ＩＬ－６ｍＲＮＡ表达升高的时相晚，且与血浆ＴＮＦα浓度变化不一致。结果表明，小肠是肠缺血－再灌流后较早产生ＴＮＦα和ＩＬ－６的部位。提示小肠在肠缺血－再灌流后在全身性炎症反应综合征的启动中可能起重要作用；血中ＴＮＦα浓度不能完全反映不同组织ＴＮＦα的含量，组织局部ＴＮＦα的增加可能是介导组织损伤的机制之一。
The expression of TNFα and IL-6mRNA in small intestine, liver and lung during intestinal ischemia-reperfusion was observed. The expression of TNFα and IL-6mRNA in small intestine began to increase 1h after intestinal ischemia, and 0.5-1h after reperfusion The results showed that the expression of TNFα and IL-6 mRNA in the liver and lung tissues were late, and the concentrations of TNFα and IL-6 were not consistent with those in the plasma. The results showed that the small intestine was the site where TNFα and IL-6 were produced earlier after intestinal ischemia-reperfusion. It is suggested that the intestine may play an important role in the initiation of systemic inflammatory response syndrome after intestinal ischemia-reperfusion. The concentration of TNFα in blood can not completely reflect the content of TNFα in different tissues. The increase of local TNFα may be mediated by tissue injury One of the mechanisms.