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本文报告了人体外周血 Tγ细胞的检测方法,并对 T 细胞分离纯化和 IgG—Fc 受体检测的一些影响因素进行了初步探讨。实验结果表明用 E_(AET)花环形成和比重1.085的 F—H液二次重复密度梯度离心组分 T 细胞,可取得较好的分离效果。用亚凝集价 IgG 抗体致敏 OxRBC 并在4℃孵育1小时可获得稳定而较高的 IgG—Fc 受体检出率。用本法检测36例正常人外周血 Tγ细胞百分率为14.9±2.7％。64例活动性肺结核患者 Tγ细胞比例为19.7±5.5％,显著高于正常人。好转期患者 Tγ细胞比例略低于进展期患者,但差别无统计学意义。对结核病患者Tγ细胞改变的意义进行了简要讨论。
This article reports the detection of human peripheral blood Tγ cells, and discusses the factors that affect the isolation and purification of T cells and the detection of IgG-Fc receptors. The experimental results show that the separation efficiency of the T cells from the F-H liquid double repeat density gradient centrifugation of E_ (AET) garland and specific gravity 1.085 can be obtained. Oxidation of OxRBCs with sub-aggregated IgG antibodies and incubation for 1 hour at 4 ° C yielded a steady and high detection rate of IgG-Fc receptors. The percentage of Tγ cells in peripheral blood of 36 healthy people tested by this method was 14.9 ± 2.7%. The proportion of Tγ cells in 64 patients with active tuberculosis was 19.7 ± 5.5%, which was significantly higher than that in normal people. T γ cells in patients with transitional stage slightly lower than the progress of patients, but the difference was not statistically significant. The significance of Tγ cell changes in patients with tuberculosis was briefly discussed.