论文部分内容阅读
为了探讨内皮素(ET)在低氧性肺动脉高 压发病中的作用和评价ETA受体拮抗 剂的预防效应，将30只Wistar大鼠分为对照组、低氧组和低氧＋BQ-123组，以常压低氧复 制 大鼠肺动脉高压模型，采用微导管法测定肺动脉平均压，放射免疫法检测血浆ET-1含量， 对 肺组织切片进行图象分析。结果发现：低氧3周后，大鼠形成明显的肺动脉高压、肺小动脉 管壁增厚和右心室肥厚，WT%和WA%均明显升高，低氧大鼠血浆ET-1含量为192.3±43.1pg/m l，明显高于对照组的128.2±28.1pg/ml (P＜0.01)；经BQ－123处理，可明显减轻低氧 所致的 肺动脉压升高、肺血管壁增厚和右心室肥厚。提示慢性低氧可导致肺动脉高压和血浆ET-1 水平升高，ETA受体拮抗剂对低氧性肺动脉高压具有显著的预防效应。
In order to investigate the role of endothelin (ET) in the pathogenesis of hypoxic pulmonary hypertension and to evaluate the preventive effect of ETA receptor antagonists, 30 Wistar rats were divided into control group, hypoxia group and hypoxia + BQ-123 group. Pulmonary hypertension was induced by normobaric hypoxia. Pulmonary artery pressure was measured by microcatheter method. Plasma ET-1 level was measured by radioimmunoassay. Image analysis of lung tissue sections was performed. The results showed that: 3 weeks after hypoxia, the rats developed obvious pulmonary hypertension, pulmonary wall thickening and right ventricular hypertrophy, WT% and WA% were significantly increased, the plasma ET-1 content of hypoxic rats was 192.3 ± 43.1 pg / ml, which was significantly higher than 128.2 ± 28.1 pg / ml in control group (P <0.01); BQ-123 treatment significantly reduced hypoxia induced pulmonary hypertension, pulmonary vascular wall thickening and right Ventricular hypertrophy. These results suggest that chronic hypoxia can lead to elevated pulmonary hypertension and plasma ET-1 levels, and ETA receptor antagonists have a significant preventive effect on hypoxic pulmonary hypertension.