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目的为探索Easy NAT TB-CPA结核分枝杆菌恒温扩增核酸检测方法在临床诊断中的价值。方法收集2015年5月1日-31日开封市结核病防治所门诊就诊的60例疑似肺结核者的痰标本,同时进行痰涂片、传统痰培养、荧光定量PCR和CPA检测。结果 60例患者中男性38例,女性22例,性别比为1.73∶1。CPA法检出阳性率为20.0%,与痰涂片法(11.7%)比较,差异有统计学意义(χ~2=5.000,P=0.025),与痰培养法(21.7%)、荧光定量PCR法(26.7%)比较,差异均无统计学意义(P>0.05)。结论 CPA方法不但快捷,更具有较高的灵敏度和特异性,值得在肺结核临床诊断工作中推广应用。
Objective To explore the value of Easy NAT TB-CPA Mycobacterium tuberculosis thermostable amplification nucleic acid detection in clinical diagnosis. Methods The sputum samples from 60 suspected pulmonary tuberculosis patients who were clinically diagnosed at the Tuberculosis Prevention and Control Clinic of Kaifeng City from May 1 to May 31, 2015 were collected. Sputum smear, traditional sputum culture, quantitative PCR and CPA were also performed. Results Among the 60 patients, 38 were male and 22 were female, with a sex ratio of 1.73: 1. The positive rate of CPA was 20.0%, which was significantly higher than that of sputum smear (11.7%) (χ ~ 2 = 5.000, P = 0.025) Act (26.7%), the difference was not statistically significant (P> 0.05). Conclusion The CPA method is not only fast, more sensitive and specific, it is worth to popularize and apply in clinical diagnosis of pulmonary tuberculosis.