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为了探讨高温致神经管畸形(NTDs)作用的分子机制,为预防人类NTDs的发生提供理论依据,本研究在高温致金黄地鼠NTDs模型的基础上,研究HMGB1在高温致金黄地鼠NTDs神经上皮细胞中的表达变化。将孕鼠随机分为实验组和对照组,分别于水浴处理后8、16、24、40、64 h(相当于胚龄第8、8.5、9、9.5、10.5 d)处死孕鼠,剖腹取鼠胚,制备石蜡切片,应用免疫荧光染色技术检测NTDs发生过程中HMGB1在神经上皮细胞中的表达变化。结果显示:对照组和模型组孕鼠在水浴处理后8、16、24h,HMGB1免疫阳性产物分布于鼠胚神经上皮细胞和周围间充质细胞的胞浆中;水浴后40、64 h,HMGB1表达部位出现了由胞浆向胞核的转移;高温处理后,HMGB1在实验组各期胚胎神经上皮细胞内的表达均比对照组减弱。上述结果提示,HMGB1的表达变化与神经管发育密切相关,其表达降低是高温致神经管畸形发生的重要途径之一。
In order to explore the molecular mechanism of NTDs induced by hyperthermia and to provide a theoretical basis for the prevention of human NTDs, this study investigated the effects of HMGB1 on NTDs induced by hyperthermia in NTDs Changes in the expression of cells. Pregnant mice were randomly divided into experimental group and control group, respectively, after 8,16,24,40,64 h (equivalent to 8, 8, 9, 9.5, 10.5 d) Mouse embryos were prepared paraffin sections, immunofluorescence staining technique was used to detect the expression of HMGB1 in neuroepithelial cells during the development of NTDs. The results showed that HMGB1 immunopositive products were distributed in the cytoplasm of rat embryonic neural epithelium cells and surrounding mesenchymal cells at 8, 16 and 24 hours after water bath treatment. At 40 and 64 h after water bath, HMGB1 The expression of HMGB1 in the embryonic neuroepithelial cells in the experimental group was weaker than that in the control group after high temperature treatment. The above results suggest that the expression of HMGB1 is closely related to the development of neural tube. The decrease of HMGB1 expression is one of the important pathways of neural tube defects induced by hyperthermia.