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目的:研究复方三金胶囊的体内抗肿瘤作用。方法:32只Balb/c-n/n裸小鼠,皮下接种人乳腺癌细胞悬液后,随机分为4组即复方三金胶囊高、低剂量组、环磷酰胺阳性药对照组、模型对照组,每组8只;复方三金胶囊高、低剂量组给药量为4、2g/kg/d,环磷酰胺为0.03g/kg/d,模型对照组给以等体积蒸馏水,每天给药1次,持续给药14天。观察复方三金胶囊对荷瘤小鼠移植瘤质量、瘤体积、体质量和脾脏质量的影响。HE染色观察给药后瘤组织病理变化。结果:与模型组比较,复方三金胶囊高、低剂量组具有不同程度的抑制移植瘤增长的作用,抑瘤率为30.64%和11.26%;复方三金胶囊高、低剂量组小鼠的体质量、胸腺质量及指数降低,但无显著性差异,且均明显高于环磷酰胺组。HE染色结果表明,与模型组比较,复方三金胶囊高、低剂量组瘤组织中细胞碎凋亡细胞、坏死细胞明显增多。结论:复方三金胶囊具有一定的体内抗肿瘤作用。
Objective: To study the antitumor effect of compound Sanjin capsule in vivo. Methods: Thirty-two Balb / cn / n nude mice were inoculated subcutaneously with human breast cancer cell suspension and then randomly divided into 4 groups: high and low dose Fujin Sanjin capsules, cyclophosphamide-positive control group and model control group , Each group of 8; compound Sanjin capsule high and low dose group dose of 4,2g / kg / d, cyclophosphamide 0.03g / kg / d, the model control group was given an equal volume of distilled water, administered daily 1 times, continuous administration of 14 days. To observe the effect of Fufang Sanjin Capsule on tumor weight, tumor volume, body weight and spleen weight in tumor-bearing mice. The histopathological changes of the tumor tissue were observed by HE staining. Results: Compared with the model group, Fufang Sanjin Capsule had the inhibitory effect on the growth of xenografts in the high and low dose groups, with the tumor inhibition rates of 30.64% and 11.26% Quality, thymus quality and index decreased, but no significant difference, and were significantly higher than the cyclophosphamide group. The results of HE staining showed that compared with the model group, the number of apoptotic cells and necrotic cells in the tumor cells of the compound Sanjin Capsules in high and low dose groups increased significantly. Conclusion: Compound Sanjin Capsule has a certain anti-tumor effect in vivo.