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目的建立饮用水中草甘膦的柱前衍生-高效液相色谱(HPLC)测定法。方法水样经氧化、衍生后,在DIKMAC18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇+0.05 mol/L磷酸二氢钾(pH=6.0)+四氢呋喃(体积比为45+55+2),流量为1.0 ml/min,柱温为25℃,激发波长为230 nm,发射波长为450 nm条件下,进行HPLC测定。结果该方法在0.025～1.0μg/ml的线性范围内,所得回归方程为y=1 571.69x+60.789,相关系数为0.999 2,方法定量下限为25μg/L。该方法的平均回收率为94.0%～106.0%,RSD为3.8%～4.6%。结论该方法操作简便、快速,适合于饮用水中草甘膦含量的检测。
Objective To establish a pre-column derivatization-high performance liquid chromatography (HPLC) assay for glyphosate in drinking water. Methods The water samples were oxidized and derivatized on a DIKMAC 18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase of methanol +0.05 mol / L potassium dihydrogen phosphate (pH = 6.0) + tetrahydrofuran (45 + 55 +2) at a flow rate of 1.0 ml / min, a column temperature of 25 ℃, an excitation wavelength of 230 nm and an emission wavelength of 450 nm. Results The linear regression equation was y = 1 571.69x + 60.789. The correlation coefficient was 0.999 2. The limit of quantification was 25 μg / L. The average recoveries of this method ranged from 94.0% to 106.0% and RSD from 3.8% to 4.6%. Conclusion The method is simple, rapid and suitable for the determination of glyphosate in drinking water.