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由尖孢镰刀菌甘薯专化型Fusarium oxysporum f.sp.batatas引起的甘薯蔓割病是甘薯生产上一种重要病害。本研究将带有GFP绿色荧光蛋白基因的T-DNA随机插入到蔓割病菌基因组中,获得711个突变体。经过致病力筛选,获得了3株非致病突变体,编号为50-5、55-7和103-4,其中50-5和103-4产孢能力显著下降。平板对峙试验结果表明这3株非致病突变体均可和蔓割病菌发生营养竞争作用。通过提前对感病品种“新种花”接种非致病突变体,再接种蔓割病菌,结果显示当蔓割病菌和非致病突变体孢子浓度同为5×10~5 CFU/m L时,103-4和55-7分别预处理感病品种8 h后,生防效果趋于稳定,分别为86.95%和83.80%;50-5预处理16 h后生防效果趋于稳定,为75.65%。以上结果表明,通过农杆菌转化筛选非致病突变体的方法,成功获得3株蔓割病生防菌株,为深入开展甘薯蔓割病的生物防治提供基础,也为其他尖孢镰刀菌创造非致病生防菌提供借鉴。
Fusarium oxysporum f.sp.batatas caused by Fusarium oxysporum f.sp.batatas is one of the most important diseases in sweet potato production. In this study, T-DNA with GFP green fluorescent protein gene was randomly inserted into the genome of the spore-forming bacteria to obtain 711 mutants. After virulence screening, three non-pathogenic mutants were obtained, numbered as 50-5, 55-7 and 103-4, in which 50-5 and 103-4 spore-forming ability decreased significantly. The test results of the plate standoff show that these three non-pathogenic mutants can compete nutritionally with the pathogenic bacteria. Inoculation of non-pathogenic mutants and inoculation of the pathogen of the susceptible variety “new species of flower ” showed that when the spore concentration of the mutants and non-pathogenic mutants was 5 × 10 ~ 5 CFU / m L , The biocontrol effect tended to be stable after being pretreated with 103-4 and 55-7 for 8 h, respectively, reaching 86.95% and 83.80% respectively. The biocontrol effect tended to be stable at 75-55 %. The above results show that three strains of non-pathogenic mutant of Agrobacterium were successfully obtained through Agrobacterium transformation, providing the basis for further biological control of sweet potato sprouting and other non-Fusarium pathogens Pathogenic bacteria antimicrobial provide reference.