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[Objective]To screen the optimum extraction technology of huperzine A from Huperzia serrata by enzymatic method. [Method]Using cellulase to hydrolyze the cytoderm,orthogonal test was carried out to optimize the extraction technology of huperzine A from H. serrata by enzymatic method. [Result]The optimum extraction technology of huperzine A from H. serrata by enzymatic method was a combination of 0.125 g cellulase,4.5 pH,60 ℃ enzymolysis temperature and 2.5 h enzymolysis time,under which the extraction rate of huperzine A could reach 0. 589‰. Compared with the acidsoak method,the extraction rate of huperzine A by enzymatic method increased by 40.3%. Besides,enzymolysis temperature was the biggest influence factor to the extraction of huperzine A from H. serrata by enzymatic method. [Conclusion]This method was simple, convenient and efficient,which could be used for extracting huperzine A from H. serrata.
[Objective] To screen the optimum extraction technology of huperzine A from Huperzia serrata by enzymatic method. [Method] Using cellulase to hydrolyze the cytoderm, orthogonal test was carried out to optimize the extraction technology of huperzine A from H. serrata by enzymatic method. [Result] The optimum extraction technology of huperzine A from H. serrata by enzymatic method was a combination of 0.125 g cellulase, 4.5 pH, 60 ° C enzymolysis temperature and 2.5 h enzymolysis time, under which the extraction rate of huperzine A could reach 0. 589 ‰. Compared with the acidsoak method, the extraction rate of huperzine A by enzymatic method increased by 40.3%. Moreover, enzymolysis temperature was the biggest influence factor to the extraction of huperzine A from H. serrata by enzymatic method. [Conclusion] This method was simple, convenient and efficient, which could be used for extracting huperzine A from H. serrata.