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目的:探讨硫化氢对慢性低O_2高CO_2模型大鼠学习记忆的影响。方法:经Morris水迷宫训练淘汰后的24只SD大鼠随机分为3组:正常对照(NC)组,低O_2高CO_2+生理盐水(HHSS)组,低O_2高CO_2+硫氢化钠(HHSH)组。HHSH组和HHSS组每天置常压低O_2高CO_2舱内8h,每周6d,共4周观察,4周后Morris水迷宫检查空间学习记忆变化,测定大鼠肺动脉平均压(mPAP)和右心室壁(RV)/左心室加室间隔(LV+S)比值、血浆中硫化氢浓度。结果:①HHSS组与NC组相比,mPAP和RV/(LV+S)比值升高,血浆中硫化氢浓度降低,平均逃避潜伏期和游泳总距离延长,穿越平台的次数减少(均P<0.05);②HHSH组与HHSS组相比,mPAP和RV/(LV+S)比值降低,血浆中硫化氢浓度升高,平均逃避潜伏期和游泳总距离缩短(均P<0.05)。结论:硫化氢可以改善慢性低O_2高CO_2模型大鼠的学习记忆障碍。
Objective: To investigate the effect of hydrogen sulfide on learning and memory in chronic hypoxia and hypercapnia rats. Methods: Twenty-four Sprague Dawley rats were randomly divided into three groups: normal control group (NC), low O_2 high CO_2 + saline (HHSS) group, low O_2 high CO_2 + sodium hydrosulfide (HHSH) group . The HHSH group and the HHSS group were routinely deprived of O_2 high CO_2 for 8h, 6d week for 4 weeks, and 4 weeks later, Morris water maze test was used to check the spatial learning and memory changes. The mean pulmonary arterial pressure (mPAP) and right ventricle Wall (RV) / left ventricular septum (LV + S) ratio, plasma hydrogen sulfide concentration. Results: ① Compared with NC group, the ratio of mPAP and RV / (LV + S) in HHSS group increased, the concentration of H2S in plasma decreased, the average escape latency and total swimming distance increased, while the number of RVs increased across the platform (all P <0.05) ; ② Compared with HHSS group, the ratio of mPAP and RV / (LV + S) decreased in HHSH group. The concentration of H2S in plasma increased, mean escape latency and total swimming distance shortened (both P <0.05). Conclusion: Hydrogen sulfide can improve the learning and memory impairment in chronic hypoxia and hypercapnia rats.