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探讨卡介苗(BCG)对家兔系膜增殖性肾炎(MsPGN)模型的保护作用及其机制。20只家兔随机分为A、B、C 3组,A、B两组均注射小牛血清白蛋白(BSA)等以建立MsPGN模型,C组为正常对照组。在静注BSA3周前A组注射BCG,B组注射生理盐水,10周实验结束。结果示:A组循环免疫复合物、肾小球细胞数、IgG沉积程度比B组明显减少(P<0.01),光镜和电镜下A组病变明显为轻,B组电子致密物较多。刀豆素-A诱导的T淋巴细胞转化试验示A组较B组增强(P<0.05),A、B两组24h尿蛋白、Scr和抗BSA抗体滴度无明显差异。结果表明BCG能促进CIC清除,使IC在小球沉积减少,减轻小球系膜增殖性炎症,同时能增强T细胞淋转功能。其保护机制可能是通过增强单核吞噬细胞系统吞噬功能和Ts功能实现的。
To investigate the protective effect and mechanism of BCG on mesangial proliferative glomerulonephritis (MsPGN) model in rabbits. Twenty rabbits were randomly divided into A, B and C groups. A and B groups were injected with bovine serum albumin (BSA) to establish MsPGN model. C group was normal control group. Group A was given BCG 3 weeks before BSA injection, group B was injected with saline, and the experiment was finished 10 weeks later. The results showed that: the number of circulating immune complex, the number of glomerular cells and the deposition of IgG in group A were significantly lower than those in group B (P <0.01). The lesions in group A were lighter under light microscope and electron microscope, while those in group B were more. Concanavalin-A induced T lymphocyte transformation test showed that group A was significantly higher than that of group B (P <0.05). There was no significant difference of 24h urine protein, Scr and anti-BSA antibody titers in groups A and B. The results show that BCG can promote CIC clearance, the IC deposition in the small ball to reduce mitochondrial proliferative inflammation, while enhancing T lymphocyte function. Its protective mechanism may be through enhanced mononuclear phagocyte system phagocytosis and Ts function to achieve.