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目的探讨维生素C(VitC)预防及治疗肝缺血再灌注脂质过氧化损伤的作用。方法制备家兔肝缺血再灌注模型 ,检测血浆及肝组织脂质过氧化产物丙二醛(MDA)和维生素E(VitE) ,动态观察肝形态学变化 ,并应用0.25g·kg-1VitC防治脂质过氧化损伤。结果肝缺血再灌注期间 ,MDA明显增高 ,VitE显著下降 ,肝形态学发生异常变化 ;缺血前及再灌注时应用VitC ,血、肝MDA均显著低于对照组 (P<0.01) ,VitE均明显高于对照组(P<0.01) ,肝形态学异常改变明显减轻。结论0.25g·kg-1VitC能抑制或减轻肝缺血再灌注脂质过氧化损伤。
Objective To investigate the effect of vitamin C on prevention and treatment of lipid peroxidation injury induced by hepatic ischemia-reperfusion. Methods Rabbit models of hepatic ischemia-reperfusion were prepared. Malondialdehyde (MDA) and vitamin E (LPS) were measured in plasma and liver tissues. The morphological changes of liver were observed dynamically and treated with 0.25g · kg-1VitC Lipid peroxidation injury. Results During the period of hepatic ischemia-reperfusion, the levels of MDA, VitE and the morphology of liver were significantly decreased. The levels of VitC, MDA in serum and liver before and after ischemia / reperfusion were significantly lower than those in control group (P <0.01) Were significantly higher than those in the control group (P <0.01), and the abnormal changes of liver morphology were significantly alleviated. Conclusion 0.25g · kg-1VitC can inhibit or alleviate the lipid peroxidation injury induced by hepatic ischemia-reperfusion.