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目的感染人体的博尔纳病病毒（ＢＤＶ）第一开放阅读框架（ＯＲＦⅠ）基因前部片段的检测至今尚未见报道。通过对先期实验已证实存在ＢＤＶ感染的人体标本和来源于感染动物的病毒标准品进行检测，拟了解感染人和动物的该病毒片段是否存在差异。方法用套式逆转录／多聚酶链反应（ｎｅｓｔｅｄＲＴ－ＰＣＲ）同时检测１２例ＢＤＶ感染的精神病人和健康献血者的周围血单核细胞和１００ｐｇ／μｌ的ＢＤＶ－ＭＤＣＫ标准病毒株中ＢＤＶＯＲＦⅠ（ｐ４０）基因前部片段。结果１２例人体标本的检测全部呈阴性，而病毒标准品呈阳性结果。结论该研究结果提示，感染人体的ＢＤＶ与来源于动物的ＢＤＶ相比较可能在病毒ＯＲＦⅠ基因片段的前面部分存在着较大的变异或前部片段缺失。该现象值得今后进一步研究。
The detection of the anterior segment of the first open reading frame (ORFⅠ) gene of Borna disease virus (BDV) in humans has not been reported yet. Through the preliminary experiments have confirmed the presence of BDV infection in human specimens and virus samples from infected animals were tested to understand the infection of human and animal virus fragment is there any difference. Methods BDVORFⅠ (p40) was detected by nested RT-PCR in peripheral blood mononuclear cells from 12 BDV-infected and healthy blood donors and 100 pg / μl BDV-MDCK standard strains. Gene front fragment. Results The results of 12 human specimens were all negative, while the virus standard was positive. Conclusions The results of this study suggest that there may be a large variation or a deletion of the anterior segment of the front part of the ORF I gene fragment in BDV infected with human beings compared with BDV derived from animals. This phenomenon deserves further study.