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体外分离培养SD大鼠胰岛细胞,分别或共同加入IL-1β(终浓度20u/mL)和IFN-γ(终浓度150u/mL)处理。加入11.1mM的葡萄糖刺激胰岛素释放。应用放射免疫分析法测定培养液上清中胰岛素浓度,流式细胞术检测胰岛细胞凋亡。结果:IL-1β单独作用于胰岛细胞,能抑制高糖刺激下胰岛素分泌及促进胰岛细胞凋亡。但与对照组比较差异无显著性(P>0.05);IFN-γ单独或联合IL-1β能显著抑制胰岛素分泌(P<0.05),促进胰岛细胞凋亡(P<0.05)。结论:IFN-γ和IL-β能诱导胰岛β细胞凋亡,并且有协同作用。与自身免疫性糖尿病的发生、发展有一定关系。
SD rat islet cells were isolated and cultured in vitro and treated with IL-1β (final concentration 20u / mL) and IFN-γ (final concentration 150u / mL) separately or together. 11.1 mM glucose was added to stimulate insulin release. The concentration of insulin in culture supernatant was measured by radioimmunoassay, and the apoptosis of islet cells was detected by flow cytometry. Results: IL-1β alone acts on islet cells, which can inhibit the insulin secretion under high glucose and promote the apoptosis of islet cells. (P <0.05). However, IFN-γ alone or in combination with IL-1β significantly inhibited insulin secretion (P <0.05) and promoted apoptosis of islet cells (P <0.05). Conclusion: IFN-γ and IL-β can induce pancreatic β-cell apoptosis and have synergistic effects. And autoimmune diabetes, the occurrence and development of a certain relationship.