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以前期获得的转抗虫基因cry6Aa2m大豆T4代植株4个株系为材料,采用PCR及RT-PCR检测方法进行了外源基因遗传稳定性及表达情况分析。并以受体品种(东农50)为对照,进行了产量性状、形态性状、品质性状及环境适应能力等农艺性状的调查。结果表明:cry6Aa2m基因能够在T4代转基因植株中遗传并表达;在产量性状上,除转基因株系DpC6b7单株结荚数显著低于对照外,其余3个株系的百粒重和单株结荚数与对照相比无显著差异;在形态性状上,除转基因株系DpC6b37株高显著低于对照外,其余株系的生育期、结荚习性、花色、叶形与对照也均无显著差异;在品质性状上,与对照相比,DpC6b37粗蛋白含量高2%,粗脂肪含量高5%。株系DpC6b7则粗蛋白含量高4%,粗脂肪含量低1%;在环境适应能力方面,转基因株系种子萌发率、落粒率、种子休眠期、杂草竞争性与对照均无显著差异。综合结果表明cry6Aa2m基因能够稳定遗传并表达,其中株系DpC6b4和DpC6b8所调查的农艺性状均不劣于受体品种。
Four strains of T4 generation of cry6Aa2m soybean that had been obtained previously were used as materials, and the genetic stability and expression of exogenous genes were analyzed by PCR and RT-PCR. In addition, the receptor variety (Dongnong 50) was used as a control to investigate the agronomic traits such as yield traits, morphological traits, quality traits and environmental adaptability. The results showed that the cry6Aa2m gene was inherited and expressed in T4 transgenic plants. In the yield traits, except the pod number of the transgenic line DpC6b7 was significantly lower than that of the control, There was no significant difference in the number of pod and the control; in the morphological traits, except the DpC6b37 transgenic plant line was significantly lower than the control, the other lines were no significant difference in growth period, pod habit, flower color, leaf shape and the control In the quality traits, DpC6b37 crude protein content was 2% higher and crude fat content was 5% higher than the control. The DpC6b7 strain had 4% higher crude protein content and 1% lower crude fat content. There was no significant difference in the environmental adaptability between the transgenic lines and the control in the seed germination rate, drop rate, dormancy period and weed competition. The results showed that cry6Aa2m gene can be inherited stably and expressed. The agronomic traits investigated by DpC6b4 and DpC6b8 were not inferior to that of recipient.