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采用菌丝生长速率法测定了新化合物氟醚菌酰胺(LH-2010A)对10种常见植物病原菌的毒力,同时研究了其对棉花立枯丝核菌无性繁殖过程、菌丝形态及细胞膜通透性的影响。结果表明:氟醚菌酰胺对10种病原菌均具有较强的抑菌活性,比结构相似的选择性杀菌剂氟吡菌胺具有更广的杀菌谱。氟醚菌酰胺对棉花立枯丝核菌和番茄灰霉病菌的抑菌活性最高,EC50值均小于1.00μg/mL,对其他8种病原菌的EC50值则在1.10～4.63μg/mL之间;而氟吡菌胺只对辣椒疫霉和马铃薯晚疫病菌具有较高的抑菌活性,EC50值分别为0.61和0.72μg/mL。在100.0μg/mL下,氟醚菌酰胺对棉花立枯丝核菌抑菌作用明显,能显著抑制其菌核产生和菌丝生长量,使菌丝分支增多、变短、变粗;以不同浓度的氟醚菌酰胺处理棉花立枯丝核菌时,其细胞膜的通透性随着药剂浓度的增加而显著增大,520 min时最高处理浓度下的相对渗率为77.01%。表明氟醚菌酰胺对多种植物病原菌具有较高的抑菌活性,并能明显抑制棉花立枯丝核菌的多项生理指标。
The mycelial growth rate method was used to determine the virulence of the new compound fluorooxyfostamide (LH-2010A) against 10 common plant pathogenic fungi. At the same time, the mycelial growth of Rhizoctonia solani, The impact of permeability. The results showed that the tetraflufenamid had strong antibacterial activity against 10 pathogenic bacteria, and had a broader antibacterial spectrum than the selective fungicide with similar structure. Flufenamic acid amide had the highest antibacterial activity against Rhizoctonia solani and Botrytis cinerea, with EC50 values less than 1.00μg / mL and EC50 values ranging from 1.10 to 4.63μg / mL for the other eight pathogens. Fluopicolide had higher antibacterial activities against Phytophthora capsici and Phytophthora infestans, with EC50 values of 0.61 and 0.72 μg / mL, respectively. At the concentration of 100.0μg / mL, the inhibitory effect of tetraflufenamid on Rhizoctonia solani was significant, which inhibited the production of mycelium and mycelial growth significantly, and increased, shortened and thicked the mycelium; The concentration of flufenamic acid treatment of Rhizoctonia solani, its cell membrane permeability increased with the concentration of the drug significantly increased, 520 min at the highest relative concentration of 77.01%. The fluorofenamic acid amide showed a high antibacterial activity against a variety of plant pathogens, and could significantly inhibit a number of physiological indexes of Rhizoctonia solani.