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实验以四个月龄任意性别的健康叙利亚腮鼠(Mesocricetus auratus)进行。动物在含有~(238)Pu 的 ZrO_2粒子气溶胶室内不麻醉下暴露22分钟。气溶胶的空气动力学直径中位值为2微米。吸入后肺内原始滞留~(238)Pu 量为140毫微居里。在暴露后1、24、138小时和1、3周,在无菌下摘除动物的肺叶,放于盛有 IM Herpes-缓冲盐溶液(HBS)的烧杯内,洗去无关的组织和血块,在 Na-EDTA-胰蛋白酶溶液中剪成小块,38℃下孵化15分钟,用
The experiment was conducted with healthy, four-month-old, healthy, Syrian mongoose (Mesocricetus auratus). The animals were exposed for 22 minutes without anesthesia in a ZrO 2 particle aerosol containing ~ (238) Pu. The aerosol has a median aerodynamic diameter of 2 microns. After inhalation of the original lung retention ~ (238) Pu amount of 140 nm Curie. At 1, 24, 138 hours and 1, 3 weeks after exposure, animals were aseptically removed in the lungs and placed in a beaker containing IM Herpes-buffered saline (HBS), washing away irrelevant tissues and blood clots Na-EDTA-trypsin solution cut into small pieces, incubated at 38 ℃ for 15 minutes, with